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Human c-Fos Activity Assay Kit

Human c-Fos Activity Assay Kit

Item no.	TFEH-CFOS-2
Manufacturer	Raybiotech
Amount	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Neuroscience Neural Lineage Markers Neural Transcription Factors Neural Signaling ELISA & Immunoassays ELISA Kits Neuronal Cell Markers
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Dry ice	Yes
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Shipping Condition	Dry ice
Available
Specificity	The olionucleotide/antibody pair provided in this kit recognizes human c-Fos in whole lysates and nuclear extracts.
Manufacturer - Category	ELISA Kits\|Transcription Factor Activity Assays
Shipping Temperature	Dry ice (additional shipping fee)
Storage Conditions	-20°C
Compatible Sample Types	Cell Lysates Nuclear Extracts
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Gene Symbols	FOSG0S7
Kit Components	96-well Strip Microplate pre-coated with DNA probesDN A Binding Buffer Positive Control Sample Specific Competitor DNA probe Non-specific Competitor DNA probe Assay ReagentDTTWash Buffer Primary Antibody HRP-conjugated Secondary Antibody Antibody Diluent Buffer TMB One-Step Substrate Reagent Stop Solution
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Absorbent paper Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2 h at RT or O/N at 4°C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared HRP-secondary antibody to each well. Incubate 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
UNSPSC Code	41116158
Manufacturer - Specificity	The olionucleotide/antibody pair provided in this kit recognizes human c-Fos in whole lysates and nuclear extracts.
Pathway	IGF SignalingJAK/STAT SignalingMAPK SignalingPI3K-AKT SignalingPKC SignalingTGF-beta Signaling

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

TFEH-CFOS-2.pdf

TFEH-CFOS-2-datasheet.pdf

TFEH-CFOS-2-safety-datasheet.pdf