Adiponutrin ELISA Kit

ELISA Kits|Competitive ELISA

Blue ice

RayBio® Human/Mouse/Rat Adiponutrin EIA Kit optimized for serum and cell culture medium.. Competition-based ELISA on a 96-well strip plate.

Competition-based

Quantitative

PNPLA3, ADPN, C22ORF20

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Sigma
Plot software (or other software that can perform four-parameter logistic regression models)

41116158

This kit detects both long form (481aa) and short form (477aa) Adiponutrin proteins. No other active isoforms have been reported. Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Human Adiponutrin (ADPN) is a 53 kD adipokine protein belong to the PNPLA family of phospholipase A2 enzymes. The 481 amino acid human Adiponutrin is a type II transmembrane protein, consisting of the patatin domain (aa 10 - 179) with enzyme activity and a transmembrane segment (aa 42 - 62). Human and mouse adiponutrins share 61% homology in amino acid sequence. Adiponutrin is mainly expressed in adipocytes and serves as a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. Reports have shown that Adiponutrin plays an important role in the balance of energy usage/storage in adipocytes. Its expression level is enhanced in response to insulin. Studies by Dr Clement's group have shown that in obese subjects, short- or long-term calorie restriction diets were associated with a reduction in Adiponutrin gene expression. Adiponutrin mRNA level was previously shown to be negatively correlated with fasting glucose plasma levels and associated with insulin sensitivity of non-diabetic obese and non-obese subjects. Reports from the same group have also shown a strong relationship between Adiponutrin expression, insulin sensitivity and glucose metabolism in human adipose tissue.Other names for Adiponutrin include PNPLA3 (patatin-like phospholipase domain-containing protein 3).RayBio® Adiponutrin EIA kit targets the common sequence of human, mouse, and rat. This kit may be used to detect Adiponutrin expression in all these species with high specificity and sensitivity.