Pro-Pancreatic Polypeptide / Icosapeptide ELISA Kit

ELISA Kits|Competitive ELISA

Blue ice

RayBio® Human/Mouse/Rat ProPPY/PI EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Competition-based

Quantitative

PPY, PNP

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Sigma
Plot software (or other software that can perform four-parameter logistic regression models)

41116158

This EIA kit is designed to detect human, mouse, and rat pro-pancreatic polypeptide (Pro-PPY) and pancreatic icosapeptide, not active pancreatic polypeptide.

The PPY gene encodes a short protein precursor that is cleaved into Pancreatic polypeptide (PP), pancreatic icosapeptide (PI) and a 5- to 7- amino-acid oligopeptide. Pancreatic polypeptide (PP) is a polypeptide secreted by PP cells in the endocrine pancreas predominantly in the head of the pancreas. It consists of 36 amino acids and has molecular weight about 4200 Da. Pancreatic icosapeptide consists of 20 amino acids.Its secretion in humans is increased after a protein meal, fasting, exercise, and acute hypoglycemia and is decreased by somatostatin and intravenous glucose.Plasma PP has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, peripheral administration of PP has been shown to decrease food intake in rodents. PP is secreted by PP pancreatic cells of Langerhans islets. It stimulates the gastric juice secretion, but inhibits the gastric secretion induced by pentagastrine. It is the antagonist of cholecystokinin and inhibits the pancreatic secretion which was stimulated by cholecystokinin. After the meal, PP seric concentration rises up from 8 to 10 times more
glucose and fats also induce PP's level increase, but on parenteral introduction of those substances, the level of hormones doesn't change. The administration of atropine, the vagotomy, blocks the PP's after-meal secretion. The excitation of the vagus nerve, the administration of gastrin, secretin or cholecystokinin induce PP secretion.The augmentation of PP secretion was observed in hormonal-active pancreatic tumors (insulin, glucagon), in Verner-Morrison syndrome, and in gastrinomas.The function of PI is unknown. The presence of porcine PI in sera is a marker of graft survival of fetal porcine pancreatic tissue in diabetic immunocompetent mice in the first 2 weeks after transplantation.This EIA kit is designed to detect pro-pancreatic polypeptide (Pro-PPY) and pancreatic icosapeptide.