Comparison

PTH ELISA Kit

Item no. EIA-PTH-5
Manufacturer Raybiotech
Amount 5 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category
Type Elisa-Kit
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Hamster - Armenian
Sensitivity 1.27 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Similar products PTH
Shipping Condition Cool pack
Available
Specificity Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Manufacturer - Category
ELISA Kits|Competitive ELISA (EIA)
Shipping Temperature
Blue ice
Storage Conditions
-20°C
Compatible Sample Types
Cell Culture Supernatants
Serum
Design Principle
Competition-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
PTH
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
HRP-Streptavidin
TMB One-Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Sigma
Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Protein Name & Synonyms
Parathyroid hormone (PTH) (Parathormone) (Parathyrin)
UNSPSC Code
41116158
Manufacturer - Specificity
Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Recommended Dilution
Human: 2X / Mouse: 2X / Rat: 2X
Introduction
Parathyroid hormone (PTH) is secreted by the chief cells of the parathyroid glands as a polypeptide containing 84 amino acids with molecular weight of 9.4KDa. PTH acts to increase the concentration of calcium (Ca2+) in the blood by acting upon parathyroid hormone receptor in three parts of the body: bone, kidney and intestine. In addition to regulation of serum calcium levels, PTH also plays role in regulating serum phosphate and vitamin D synthesis. PTH reduces the reabsorption of phosphate from the proximal tubule of the kidney, resulting in increased phosphate excretion through the urine. However, PTH enhances the uptake of phosphate from the intestine and bones into the blood. In the bone, slightly more calcium than phosphate is released from the breakdown of bone. In the intestines, which are mediated by an increase in activated vitamin D, the absorption of phosphate is not as dependent on vitamin D as is that of calcium. The end result is a small net drop in the serum concentration of phosphate. PTH increases the activity of 1-alpha-hydroxylase enzyme, which converts 25-hydroxycholecalciferol to 1, 25-dihydroxycholecalciferol, the active form of vitamin D.PTH has shown significant clinical applications. A high level of PTH in the blood is known as hyperparathyroidism. The causes of primary hyperparathyroidism are parathyroid adenoma, parathyroid hyperplasia and parathyroid cancer. The cause for secondary hyperparathyroidism is often due to in chronic renal failure.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 Plate Kit
Available: In stock
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