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Vaspin ELISA Kit

Vaspin ELISA Kit

Item no.	EIA-VAP-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	26.2 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	Vaspin
Shipping Condition	Cool pack
Available
Manufacturer - Category	ELISA Kits\|Competitive ELISA
Manufacturer - Targets	Serpin A12, Vaspin
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	RayBio® Human/Mouse/Rat Vaspin EIA Kit for serum, culture supernatants and cell lysates. Competition-based ELISA on a 96-well strip plate. Optimized for serum and cell culture medium.
Compatible Sample Types	Plasma, Serum
Design Principle	Competition-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	SERPINA12
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Standard Peptide Assay Diluent(s) Biotinylated Peptide HRP-Streptavidin TMB One-Step Substrate Stop Solution Assay Diagram Positive Control Sample Capture Antibody User Manual
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Orbital shaker Aluminum foil Saran Wrap Absorbent paper Microplate reader capable of measuring absorbance at 450nm Sigma Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline	Prepare all reagents, samples and standards as instructed. Add 100 µl detection antibody to each well. Incubate 1.5 h at RT or O/N at 4°C. Add 100 µl standard or sample to each well. Incubate 2.5 h at RT. Add 100 µl prepared streptavidin solution. Incubate 45 min at RT. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl Stop Solution to each well. Read plate at 450 nm immediately.
UNSPSC Code	41116158
Protein Name & Synonyms	Serpin A12 (OL-64) (Visceral adipose tissue-derived serine protease inhibitor) (Vaspin) (Visceral adipose-specific serpin)
Manufacturer - Specificity	Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Recommended Dilution	Human: 2X / Mouse: 2X / Rat: 2X
Introduction	Visceral adipose tissue-derived serpin (Vaspin), a member of serine protease inhibitor family, is a visceral adipose tissue-derived adipokine with potential antiprotease properties. Vaspin cDNA was isolated by from visceral white adipose tissues (WATs) of Otsuka Long-Evans Tokushima fatty (OLETF) rat, an animal model of abdominal obesity with type 2 diabetes. Human, mouse and rat Vaspin is made up of 395, 394, and 392 amino acids respectively. Vaspin belongs to serine protease inhibitor family and it also shows about 40% homology with 1-antitrypsin.Vaspin ameliorates certain aberrations seen in the diabetic obesity metabolic syndrome by sensitizing insulin action, especially in WATs. Research on Vaspin has been trying for the identification of the potential protease substrate leading to the development of antiprotease inhibitor therapy, which could facilitate the improvement of insulin sensitivity in this metabolic syndrome.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

EIA-VAP-1.pdf

EIA-VAP-1-datasheet.pdf

EIA-VAP-1-safety-datasheet.pdf