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Human G-CSF ELISA Kit

Human G-CSF ELISA Kit

Item no.	ELH-GCSF-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	1 pg/ml
Citations	1. Moura RR, Albuquerque ES, Henrique C, et al. Dynamics of Recombinant hG-CSF in Transgenic Goat: Preliminary Study in the Founder during Hormonally Induced Lactation. Anim Biotechnol. 2013,274(1):10–14. 2. Nair N., Chidambareswaren M., Manjula S. Enhanced Heterologous Expression of Biologically Active Human Granulocyte Colony Stimulating Factor in Transgenic Tobacco BY-2 Cells by Localization to Endoplasmic Reticulum. Molecular Biotechnology, 2014 Epub Ahead of Print. DOI 10.1007/s12033-014-9765-x 3. Batista R., et al. Phenotypic features of first-generation transgenic goats for human granulocyte-colony stimulation factor production in milk. Biotechnology Letters Online First June 2014. DOI 10.1007/s10529-014-1588-0 4. El-Ganzoury M., et al. Enteral Granulocyte-Colony Stimulating Factor and Erythropoietin Early in Life Improves Feeding Tolerance in Preterm Infants: A Randomized Controlled Trial. The Journal of Pediatrics, Aug 2014, epub ahead of print. DOI: 10.1016/j.jpeds.2014.07.034 5. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265 6. Lee, YH., Shynlova, O., et al. Stretch-induced human myometrial cytokines enhance immune cell recruitment via endothelial activation. Cellular & Molecular Immunology 2014. Epub Ahead of Print DOI: doi: 10.1038/cmi.2014.39
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	G-CSF
Shipping Condition	Cool pack
Available
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	GCSF
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Human GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	CSF3, C17ORF33, GCSF
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Granulocyte colony-stimulating factor (G-CSF) (Pluripoietin) (Filgrastim) (Lenograstim)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Recommended Dilution	2 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELH-GCSF-5.pdf

ELH-GCSF-5-datasheet.pdf

ELH-GCSF-5-safety-datasheet.pdf