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Human Leptin ELISA Kit

Human Leptin ELISA Kit

Item no.	ELH-Leptin-2
Manufacturer	Raybiotech
Amount	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Obesity ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Breast Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	2 pg/ml
Citations	1. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-alpha on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006,452:418–427 2. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541. doi:10.1371/journal.pone.0058541 3. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541. 4. Associations between perinatal factors and adiponectin and leptin in 9-year-old Mexican-American children 5. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541. doi:10.1371/journal.pone.0058541 6. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013,8(3):e58541. 7. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print] 8. Bil-Lula I., et al. Is There any Link Between Visceral Obesity and Adenovirus Infections in the Polish Population? Adv Clin Exp Med 2014, 23, 3, 415–422 9. Bhaktha G., et al. SERUM TNF-alpha AND LEPTIN LEVELS IN GRADED OBESE DIABETIC WOMEN- FREE FROM MICRO AND MACRO VASCULAR COMPLICATIONS. IJPCBS 2014, 4(3), 648-653 10. Ha AW., et al. Plasma adipocytokines and antioxidants-status in Korean overweight and obese females with dyslipidemia. Nutr Res Pract. 2014 Aug,8(4):417-424. http://dx.doi.org/10.4162/nrp.2014.8.4.417 11. Lin Y., et al. Dietary fiber intake and its association with indicators of adiposity and serum biomarkers in European adolescents: the HELENA study. European Journal of Nutrition August 2014, Epub Ahead of Print. DOI 10.1007/s00394-014-0756-2 12. Bil-lula I., et al. Infectobesity in the Polish Population - Evaluation of an Association between Adenoviruses Type 5, 31, 36 and Human Obesity. International Journal of Virology and Molecular Biology 2014, 3(1): 1-8. DOI: 10.5923/j.ijvmb.20140301.01
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	Leptin
Shipping Condition	Cool pack
Available
Specificity	The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	Leptin
Shipping Temperature	Blue ice
Storage Conditions	?-20°C
Description	RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Solid Support	96-well Microplate
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Result Output	Quantitative
UNSPSC Code	41116158
Manufacturer - Specificity	The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Recommended Dilution	20-50 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELH-Leptin-2.pdf

ELH-Leptin-2-datasheet.pdf

ELH-Leptin-2-safety-datasheet.pdf