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Human Osteoprotegerin (OPG) ELISA Kit

Human Osteoprotegerin (OPG) ELISA Kit

Item no.	ELH-OPG-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Metabolism Glucose Homeostasis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Thyroid Cancer Brain Cancer Nervous System Cancer Lymphatic System Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	1 pg/ml
Citations	1. Mohamed JA, DuPont HL, Jiang ZD, Flores J, Carlin LG, Belkind-Gerson J, et al. A Single-Nucleotide Polymorphism in the Gene Encoding Osteoprotegerin, an Anti-inflammatory Protein Produced in Response to Infection with Diarrheagenic Escherichia coli, Is Associated with an Increased Risk of Nonsecretory Bacterial Diarrhea in North American Travelers to Mexico. J Infect Dis. 2009,199:477–485. 2. Maksymowych WP, Landewe R, Conner-Spady B, Dougados, et al. Serum Matrix Metalloproteinase 3 Is an Independent Predictor of Structural Damage Progression in Patients with Ankylosing Spondylitis. Arthritis Rheum. 2007,56(6):1846–1853. 3. Jose´ I Botella-Carretero, Francisco Alvarez-Blasco, Jose´ Luis San Milla´n and He´ctor F Escobar-Morreale. Thyroid hormone deficiency and postmenopausal status independently increase serum osteoprotegerin concentrations in women. Eur J Endocrinol. 2007,156:539–545 4. Nishigaki M, Yamamoto T, Ichioka H, et al. beta-cryptoxanthin regulates bone resorption related-cytokine production in human periodontal ligament cells. Arch Oral Biol. 2013 Jul,58(7):880–886. 5. Akelma AZ, Cizmeci MN, Kanburoglu MK, Bozhkaya D, Catal F, et al. Elevated level of serum osteopontin in school-age children with asthma. Allergol Immunop (Madr). 2013, Published online ahead of print, April 8, 2013. doi:10.1016/j.aller.2013.01.009 6. Pamuk BO, Sari I, Selcuk S, et al. Evaluation of circulating endothelial biomarkers in familial Mediterranean fever. Rheumatol Int. 2013 Aug,33(8):1967–1972. 7. Hartwich JE, Orr WS, Ng CY, et al. Rapamycin increases neuroblastoma xenograft and host stromal derived osteoprotegerin inhibiting osteolytic bone disease in a bone metastasis model. J Pediatr Surg. 2013 Jan,48(1):47–55. 8. Lucas R., Ramos E., Prata M., Rodrigues A., Costa L., et al. Changes in serum RANKL and OPG with sexual development and their associations with bone turnover and bone mineral density in a cohort of girls. Clinical Biochemistry Available online 22 April 2014 [Epub ahead of Print] 9. Palumbo S, Li W-J. Osteoprotegerin Enhances Osteogenesis of Human Mesenchymal Stem Cells. Tissue Eng Part A. Volume 19, Numbers 19 and 20, 2013, DOI: 10.1089/ten.tea.2012.0550 10. Holt V, Caplan AI, Haynesworth SE (2014) Identification of a Subpopulation of Marrow MSC-Derived Medullary Adipocytes That Express Osteoclast- Regulating Molecules: Marrow Adipocytes Express Osteoclast Mediators. PLoS ONE 9(10): e108920. doi:10.1371/journal.pone.0108920
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	Osteoprotegerin, TNFRSF11B
Shipping Condition	Cool pack
Available
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF,
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	Osteoprotegerin, TNFRSF11B
Shipping Temperature	Blue ice
Storage Conditions	?-20°C
Description	RayBio® Human Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Solid Support	96-well Microplate
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Result Output	Quantitative
UNSPSC Code	41116158
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Recommended Dilution	3 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELH-OPG-5.pdf

ELH-OPG-5-datasheet.pdf

ELH-OPG-5-safety-datasheet.pdf