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Human Phosphotyrosine SCFR ELISA

Human Phosphotyrosine SCFR ELISA

Manufacturer	Raybiotech
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
Amount	5 Plate Kit
Item no.	PEL-SCFR-Y-5
Targets	CD117
eClass 6.1	32160605
eClass 9.0	32160605
Available
Quantity options
Compatible Sample Types	Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Biotinylated Anti-Phosphotyrosine Antibody Stop Solution Assay Diluent(s) Positive Control Sample Lysis Buffer Streptavidin-Conjugated HRP TMB One-Step Substrate
Method of Detection	Colorimetric
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 ul to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Product Features	Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
Protocol Summary	Prepare all reagents and samples as instructed in the manual. Add 100 ul of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 C. Add 100 ul of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Result Output	Semi-Quantitative
Short description	RayBio Custom Human Phosphotyrosine SCFR ELISA Kit (Available only on demand)
Solid Support	96-well Microplate
Storage	Upon receipt, the kit should be stored at -20 C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 C. Reconstituted Positive Control (Item K) should be stored at -70 C.
Target Name and Synonyms	c-kit (P-Tyr), CD117 (P-Tyr), SCF R (P-Tyr)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.