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Mouse TNF-alpha ELISA Kit

Mouse TNF-alpha ELISA Kit

Item no.	ELM-TNFa-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Testicular & Prostate Cancer
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Sensitivity	60 pg/ml (sensitivity of lysate is 94 pg/ml)
Citations	1. Cheng C-Y, Sue Y-M, Chen C-H, Hou C-C, Chan P, et al. Tetramethylpyrazine Attenuates Adriamycin-Induced Apoptotic Injury in Rat Renal Tubular Cells NRK-52E. Planta Med. 2006,72:888–893. 2. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gsalpha-dependent signaling. Blood. 2013,121(6):930–939. 3. Erythropoietin inhibits gluconeogenesis and inflammation in the liver and improves glucose intolerance in high-fat diet-fed mice 4. Meng R, Zhu D, Bi Y, et al. Erythropoietin inhibits gluconeogenesis and inflammation in the liver and improves glucose intolerance in high-fat diet-fed mice. PLoS ONE. 2013,8(1):e53557. 5. Meng R, Zhu D, Bi Y, et al. Erythropoietin inhibits gluconeogenesis and inflammation in the liver and improves glucose intolerance in high-fat diet-fed mice. PLoS ONE. 2013,8(1):e53557. 6. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1, 123(2): 751–766. 7. Kabel AM, Abdel-Rahman MN, El-Sisi AE-DE, et al. Effect of atorvastatin and methotrexate on solid Ehrlich tumor. Eur J Pharmacol. 2013 May 10,713(1-3):47–53. 8. Shi Q., Cao J., Fang L., Zhao., Liu Z., Ran., et al. Geniposide suppresses LPS-induced nitric oxide, PGE 2 and inflammatory cytokine by downregulating NF-kappaB, MAPK and AP-1 signaling pathways in macrophages. Int Immunopharmacol 2014 Apr 13,20(2):298-306. Epub 2014 Apr 13. 9. Morganti J., Jopson T., Liu S., Gupta N., Rosi S. Cranial Irradiation Alters the Brain’s Microenvironment and Permits CCR2+ Macrophage Infiltration. PLOS One Published: April 02, 2014 DOI: 10.1371/journal.pone.0093650 [Epub Ahead of Print] 10. Bishayi B., Bandyopadhyay D., Majhi A., Adhikary R. POSSIBLE ROLE OF TOLL LIKE RECEPTOR-2 (TLR-2) IN THE INTRACELLULAR SURVIVAL OF STAPHYLOCOCCUS AUREUS IN MURINE PERITONEAL MACROPHAGES: INVOLVEMENT OF CYTOKINES AND ANTI-OXIDANT ENZYMES. Scandinavian Journal of Immunology. 2014, May Epub Ahead of Print, doi:10.1111/sji.12195 11. Zehntner, SP et al. Neutrophils that infiltrate the central nervous system regulate T cell responses. Neutrophils that infiltrate the central nervous system regulate T cell responses. Neutrophils that infiltrate the central nervous system regulate T cell responses. J. Immunol. Volume 174, Issue 8, Pages 5124-31, Apr 2005 12. Zhang Y., Tang Y., Chen B., Valeriote F., Li X., Shaw J. UTL-5g Lowers Elevated Blood Levels of TNF-? and TGF-beta and Increases Survival Rates in Animals Treated with LPS/D-(+)-galactosamine. Am. J. Biomed. Sci. 2014, 6(2), 128-138, doi: 10.5099/aj140200128 13. Majhi A., Ashikary R., Bhattacharyya A., Mahanti S., Bishayi B. LEVOFLOXACIN AND CEFTRIAXONE IN COMBINATION ATTENUATES LUNG INFLAMMATION IN A MOUSE MODEL OF BACTEREMIC PNEUMONIA BY MULTI-DRUG RESISTANT Streptococcus pneumoniae VIA INHIBITION OF CYTOLYTIC ACTIVITIES OF PNEUMOLYSIN AND AUTOLYSIN. Antimicrob. Agents Chemother. Accepted ahead of Print, June 23 2014. doi:10.1128/AAC.03245-14 14. Bil-Lula I., et al. Adenovirus type 9 enhances differentiation and decreases cytokine release from preadipocytes. J of Med Vir Article first published online: 30 JUN 2014 DOI: 10.1002/jmv.24009 15. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-Induced Acute Lung Inflammation Through Inhibition of NF-kappab, STAT3 and Pro-Inflammatory Mediators and Enhancement of IkappaBalpha and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y 16. Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314 17. Yu L., et al. MRTF-A mediates LPS-induced pro-inflammatory transcription by interacting with the COMPASS complex. Journal of Cell Science. Advance Online Article September 4, 2014 doi: 10.1242/jcs.152314 18. Moulari, B., Beduneau, A. et al. Lectin-decorated nanoparticles enhance binding to the inflamed tissue in experimental colitis. Journal of Controlled Release 2014. Epub Ahead of Print. DOI: 10.1016/j.jconrel.2014.05.046
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	TNF alpha
Shipping Condition	Cool pack
Available
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	TNF alpha
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Mouse TNF alpha ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	TNF, TNFSF2
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Recommended Dilution	2 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELM-TNFa-1.pdf

ELM-TNFa-1-datasheet.pdf

ELM-TNFa-1-safety-datasheet.pdf