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Mouse VEGF ELISA Kit

Mouse VEGF ELISA Kit

Item no.	ELM-VEGF-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology By Cell Type Carcinoma Cell Biology Cell Culture Cytokines Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Head & Neck Cancer Testicular & Prostate Cancer Brain Cancer Nervous System Cancer Hepatic Cancer
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Sensitivity	2 pg/ml
Citations	1. Saldaña L, Crespo L, Bensiamar F, et al. Mechanical forces regulate stem cell response to surface topography. J Biomed Mater Res A. 2013 Apr 24, [Epub ahead of print] doi:10.1002/jbm.a.34674. 2. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1, 123(2): 751–766. 3. Xiao X, Guo P, Chen Z, et al. Hypoglycemia reduces vascular endothelial growth factor A production by pancreatic beta cells as a regulator of beta cell mass. J Biol Chem. 2013 Mar 22,288(12):8636–8646. 4. Martin D, Nguyen Q, Molinolo A, Gutkind JS. Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene. Oncogene. 2013 May 27, [Epub ahead of print] doi: 10.1038/onc.2013.193. 5. Hartwich J, Orr WS, Ng CY, et al. HIF-1alpha activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan,48(1):39–46. 6. Hartwich J, Orr WS, Ng CY, et al. HIF-1alpha activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan,48(1):39–46. 7. Bhuvaneswari R, Gan Y, Yee K, Soo K,Olivo M. Effect of hypericin-mediated photodynamic therapy on the expression of vascular endothelial growth factor in human nasopharyngeal carcinoma. Int J Mol Med. 2007,20:421-428. 8. Nasr M., Nafee N., Saad H., Kazem A. Improved antitumor activity and reduced cardiotoxicity of epirubicin using hepatocyte-targeted nanoparticles combined with tocotrienols against hepatocellular carcinoma in mice. European Journal of Pharmaceutics and Biopharmaceutics. Available online 6 May 2014, http://dx.doi.org.proxy.library.emory.edu/10.1016/j.ejpb.2014.04.016 [Epub ahead of print] 9. Hartwich J., Orr S., Ng C., Spence Y., Morton C., DavidoffA. HIF-1[alpha] activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. Journal of Pediatric Surgery Volume 48, Issue 1 , Pages 39-46, January 2013 10. Konac E., et al. Does Pattern Scan Laser (PASCAL) photocoagulation really induce less VEGF expression in murine retina than conventional laser treatment? Gene. In Press, Corrected Proof, Available online 24 July 2014. http://dx.doi.org/10.1016/j.gene.2014.07.062 11. Abbas OL., et al. Inhibition of the Notch Pathway Promotes Flap Survival by Inducing Functional Neoangiogenesis. Annals of Plastic Surgery August 28, 2014, epub ahead of print. doi: 10.1097/SAP.0000000000000197 12. Wei, Z., Yu, S. et al. Regulatory Role of the JNK-STAT1/3 Signaling in Neuronal Differentiation of Cultured Mouse Embryonic Stem Cells. Cellular and Molecular Neurobiology 2014. Epub Ahead of Print: DOI: 10.1007/s10571-014-0067-4
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	VEGF-A
Shipping Condition	Cool pack
Available
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	VEGF-A
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	VEGFA, VEGF
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Recommended Dilution	3 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELM-VEGF-5.pdf

ELM-VEGF-5-datasheet.pdf

ELM-VEGF-5-safety-datasheet.pdf