Comparison

Custom Human Phospho-APP (Thr668) and Total APP Cell-Based ELISA Kit

Item no. CBEL-APP-T668-5
Manufacturer Raybiotech
Amount 5 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category
Type Elisa-Kit
Specific against Human (Homo sapiens)
Host Hamster - Armenian
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Shipping Condition Cool pack
Available
Specificity This ELISA kit recognizes Human APP phosphorylated at site Threonine-668 as well as total APP.
Manufacturer - Category
Assay Kits|Phosphorylation Assays|ELISA Kits|Cell-Based ELISA
Shipping Temperature
Blue ice
Storage Conditions
-20°C
Compatible Sample Types
Adherent Cell Culture
Design Principle
Cell-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
96-well Microplate
Gene Symbols
A4AD1APP
Kit Components
Uncoated 96-well Strip Microplate
Wash Buffers
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution
Other Materials Required
Distilled or deionized water 100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
Seed 10, 000-30, 000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1
X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
Add 50 µl of 1
X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1
X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
UNSPSC Code
41116158
Protein Name & Synonyms
Amyloid beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP Soluble APP-alpha (S-APP-alpha) Soluble APP-beta (S-APP-beta) C99 Beta-amyloid protein 42 (Beta-APP42) Beta-amyloid protein 40 (Beta-APP40) C83 P3(42) P3(40) C80 Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)) Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)) Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)) C31]
Manufacturer - Specificity
This ELISA kit recognizes Human APP phosphorylated at site Threonine-668 as well as total APP.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 Plate Kit
Available: In stock
available

Delivery expected until 8/28/2025 

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