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Human Phospho-BTK (Y551) Quantitative ELISA Kit

Human Phospho-BTK (Y551) Quantitative ELISA Kit

Item no.	PEL-BTK-Y551-Q-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology By Cell Type Hematology Leukemia Lymphoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Phosphorylation ELISA
Manufacturer - Targets	BTK
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	RayBio® Human Phospho-BTK (Y551) Quantitative ELISA Kit. This assay quantitatively measures phosphorylated BTK (Tyr551) in lysate samples.
Compatible Sample Types	Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	BTK, AGMX1, ATK, BPK
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Standard Protein
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or standard to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1 X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
UNSPSC Code	41116158
Protein Name & Synonyms	Tyrosine-protein kinase BTK (EC 2.7.10.2) (Agammaglobulinemia tyrosine kinase) (ATK) (B-cell progenitor kinase) (BPK) (Bruton tyrosine kinase)
Manufacturer - Specificity	The antibody pair provided in this kit recognizes human BTK phosphorylated at site Tyrosine-551.
Pathway	B Cell ReceptorTyrosine Kinase Family
Introduction	RayBio® Phospho-BTK (Y551) Quantitative ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated BTK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human phospho-BTK. An anti-pan BTK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and BTK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-BTK (Y551) antibody is used to detect phosphorylated BTK. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BTK (Y551) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Stimulation	THP-1 cells were treated or untreated with Pervanadate for 10 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.