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Human Phospho-RB (T826) and Total RB ELISA Kit

Human Phospho-RB (T826) and Total RB ELISA Kit

Item no.	PEL-RB-T826-T-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status ELISA Senescence ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma By Organ Bladder Cancer Bone Cancer
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Specificity	This ELISA kit recognizes Human RB phosphorylated at site Threonine-826 as well as total RB.
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Phosphorylation ELISA
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Gene Symbols	RB1
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1 X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Retinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110)
UNSPSC Code	41116158
Manufacturer - Specificity	This ELISA kit recognizes Human RB phosphorylated at site Threonine-826 as well as total RB.
Positive Control	HT29 cells were treated with Thymidine and Nocodazole. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
Stimulation	HT29 cells were treated with Thymidine-Nocodazole Block. Cell lysates were analyzed using this phosphoELISA and Western Blot.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

PEL-RB-T826-T-5.pdf

PEL-RB-T826-T-5-datasheet.pdf

PEL-RB-T826-T-5-safety-datasheet.pdf