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Rat MCP-1 ELISA Kit

Rat MCP-1 ELISA Kit

Item no.	ELR-MCP1-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Neuroscience Neuroinflammation ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	15 pg/ml
Citations	1. Omi H., et al. Low-intensity pulsed ultrasound stimulation enhances TIMP-1 in nucleus pulposus cells and MCP-1 in macrophages in the rat. J Orthop Res. 2008 Jun,26(6):865-71. doi: 10.1002/jor.20545. 2. Ergenoglu AM, Yeniel AÖ, Erbas O, et al. Regression of Endometrial Implants by Resveratrol in an Experimentally Induced Endometriosis Model in Rats. Reprod Sci. 2013, Published online before print March 27, 2013, doi:10.1177/1933719113483014 3. Ergenoglu AM, Yeniel AÖ, Erbas O, et al. Regression of Endometrial Implants by Resveratrol in an Experimentally Induced Endometriosis Model in Rats. Reprod Sci. 2013, Published online before print March 27, 2013, doi:10.1177/1933719113483014 4. Savary-Auzeloux, Isabelle, Hugues Magne, and Carole Migne. "A Dietary Supplementation with Leucine and Antioxidants Is Capable to Accelerate Muscle Mass Recovery after Immobilization in Adult Rats." PLoS One. NCBI, 29 Nov. 2013. Web. 5. Smits A., et al. Shear flow affects selective monocyte recruitment into MCP-1-loaded scaffolds. J. Cell. Mol. Med, Early Online view, August 2014. doi: 10.1111/jcmm.12330
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	CCL2, MCP-1
Shipping Condition	Cool pack
Available
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	MCP-1 / CCL2
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Rat MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	CCL2, SCYA2, MCP1, JE
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Recommended Dilution	500 - 10, 000 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELR-MCP1-5.pdf

ELR-MCP1-5-datasheet.pdf

ELR-MCP1-5-safety-datasheet.pdf