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Rat VEGF-A ELISA Kit

Rat VEGF-A ELISA Kit

Item no.	ELR-VEGF-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology By Cell Type Carcinoma Cell Biology Cell Culture Cytokines Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Bone Cancer
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	2 pg/ml
Citations	1. Ergenoglu AM, Yeniel AÖ, Erbas O, et al. Regression of Endometrial Implants by Resveratrol in an Experimentally Induced Endometriosis Model in Rats. Reprod Sci. 2013, Published online before print March 27, 2013, doi:10.1177/1933719113483014 2. Gandhi S, Srinivasan B, Akarte AS. Aliskiren improves insulin resistance and ameliorates diabetic renal vascular complications in STZ-induced diabetic rats. J Renin Angiotensin Aldosterone Syst. 2013,14(1):3–13. 3. Yüksel H, Yavuz O, Is M, et al. Simvastatin reduces VEGF and NO levels in acute stages of experimental traumatic brain injury. Neurol. Sci. 2013 Mar 31. 4. Von Dobschuetz E, Meyer S, Thorn D, Marme D, Hopt UT, Thomusch O. Targeting Vascular Endothelial Growth Factor Pathway Offers New Possibilities to Counteract Microvascular Disturbances During Ischemia/Reperfusion of the Pancreas. Transplanation. 2006,82:543–549. 5. Zhou, Bin-Quan, Dong-Mei Jiang, and Jie Han. "Paracrine Effects of Bone Marrow–Derived Endothelial Progenitor Cells: Cyclooxygenase-2/Prostacyclin Pathway in Pulmonary Arterial Hypertension." PLoS One. NCBI, 18 Nov. 2013. Web. 6. Tanishita, Kazuo, Yoshinori Abe, and Yoshiyuki Ozaki. "Endothelial Progenitor Cells Promote Directional Three-Dimensional Endothelial Network Formation by Secreting Vascular Endothelial Growth Factor." PLoS One. NCBI, 03 Dec. 2013. Web. 7. Kumai Y, Ooboshi H, Ibayashi S, Ishikawa E, et al. Postischemic gene transfer of soluble Flt-1 protects agains brain ischemia with marked attenuation of blood-brain barrier permeability. J Cerebr Blood Flow Metab. 2007, 27:1152–1160. 8. Hao C., Huang Z., Song S., Shi Y., Cheng X., Murohara T., Lu W., Su D., Duan J. Arterial Baroreflex Dysfunction Impairs Ischemia-Induced Angiogenesis .J Am Heart Assoc. 2014, 3: e000804. 9. Hao C., Huang J., Shi Y., Cheng X., Li H., Zhou L., et al. Pulsed electromagnetic field improves cardiac function in response to myocardial infarction. Am J Transl Res 2014,6(3):281-290 10. Oktem M., et al. The effect of captopril on endometriotic implants in a rat model. European Journal of Obstetrics & Gynecology and Reproductive Biology, In Press, Uncorrected Proof, Available online 19 July 2014. http://dx.doi.org/10.1016/j.ejogrb.2014.06.028 11. Oktem M., et al. The effect of captopril on endometriotic implants in a rat model. European Journal of Obstetrics & Gynecology and Reproductive Biology, In Press, Uncorrected Proof, Available online 19 July 2014. http://dx.doi.org/10.1016/j.ejogrb.2014.06.028 12. Adamek B., et al. Vascular endothelial growth factor (VEGF) liver tissue concentration in delayed stages of rat liver regeneration upon interferon a2b influence. Postepy Nauk Medycznych. t. XXVII, nr 6, 2014 13. Gandhi S., et al. Aliskiren improves insulin resistance and ameliorates diabetic renal vascular complications in STZ-induced diabetic rats. Journal of the Renin-Angiotensin-Aldosterone System. 14(1) 3–13. DOI: 10.1177/1470320312452766
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	VEGF-A
Shipping Condition	Cool pack
Available
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	VEGF-A
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Rat VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	VEGFA, VEGF
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Recommended Dilution	2 fold

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.