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Recombinant Human TNF alpha Protein

Recombinant Human TNF alpha Protein

Item no.	230-00243-100
Manufacturer	Raybiotech
Amount	100 ug
Quantity options	10 ug 100 ug 50 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Western Blot ELISA Immunostaining Dot Blot
Type	Proteins Recombinant
Format	Liquid
Applications	WB, ELISA, other, Dot
Specific against	Human (Homo sapiens)
Host	E.coli
Conjugate/Tag	HIS
Purity	> 95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	TNFa,P01375,TNFa,TNFa,TNF-alpha,Tumor necrosis factor
Similar products	TNFa
Shipping Condition	Cool pack
Available
Manufacturer - Applications	ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	C-terminal 6x histidine tag
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Molecular Weight	17 kDa
Description	Recombinant Human TNF-alpha
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	TNFA
UNSPSC Code	12352202
Formulation	Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	Val77-Leu233
Protein Name & Synonyms	TNFa, TNF-alpha, Tumor necrosis factor
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Lot specific (see the label on the vial)
Endotoxin Level	Not determined
Preservative	None
Data Images	Figure 1 Transcription factor activity assay of NF-ÎºB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-Î± (RayBio Cat# 230-00243). After stimulation with TNF-Î±, activated NF-ÎºB p65 is translocated into the nucleus where it binds with its corresponding DNA. A. Western blotting result of NF-ÎºB p65 from cytoplasm and nuclear fractions B. Transcription factor activity assay of NF-ÎºB p65 from nuclear fractions, measured by TF-ELISA kit (RayBio Cat# TFEH-p65).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

230-00243-100.pdf

230-00243-100-datasheet.pdf

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Amount: 100 ug

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Delivery expected until 12/11/2025

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