Comparison

Recombinant Human ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2)

Item no. 230-30008-10
Manufacturer Raybiotech
Amount 10 ug
Quantity options 10 ug 100 ug 50 ug
Category
Type Proteins Recombinant
Format Lyophilized
Applications WB, IP, ELISA, Dot
Specific against Human (Homo sapiens)
Host HEK293 cells
Conjugate/Tag HIS
Purity > 90%
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2,CD340,NGL,HER-2,HER2,HER-2/neu,NEU,TKR1,Neuro/Glioblastoma Derived Oncogene Homolog,C-Erb B2/Neu Protein,Metastatic Lymph Node Gene 19 Protein,herstatin,Neuroblastoma/Glioblastoma Derive
Shipping Condition Room temperature
Available
Manufacturer - Applications
ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array
Manufacturer - Category
Proteins|Recombinant Proteins|Human Proteins|Enzymes & Inhibitors
Manufacturer - Conjugate / Tag
N-terminal 9x histidine tag
Shipping Temperature
Ambient temperature
Storage Conditions
-20°C
Molecular Weight
The recombinant human ERBB2/Her2 extracellular domain has a calculated molecular mass of 71 kDa. Due to the abundant glycosylation, it migrates as an approximately 100 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Description
Recombinant Human Erb/Her2 (Receptor tyrosine-protein kinase erbB-2), N-terminal extracellular domain, derived from the transfected human HEK293 cells
Purity determined by
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols
ERBB2
UNSPSC Code
12352202
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS
Expressed Region
Thr23-Thr652
Protein Name & Synonyms
V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2, CD340, NGL, HER-2, HER2, HER-2/neu, NEU, TKR1, Neuro/Glioblastoma Derived Oncogene Homolog, C-Erb B2/Neu Protein, Metastatic Lymph Node Gene 19 Protein, herstatin, Neuroblastoma/Glioblastoma Derived Oncogene Homolog, Proto-Oncogene Neu, Receptor Tyrosine-Protein Kinase -2, Tyrosine Kinase-Type Cell Surface Receptor HER2, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog, MLN 19, MLN19, p185erbB2, CD340 Antigen, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2, Receptor tyrosine-protein kinase erbB-2, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2
Format
Lyophilized powder
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level
Not determined
Preservative
None
SDS-PAGE Image
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: Protein standard ladder (kDa).Lane 2: Untreated protein under reducing conditions.Lane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 10 ug
Available: In stock
available

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