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Human CellExp™ TFPI2, Human recombinant

Human CellExp™ TFPI2, Human recombinant

Item no.	268-11082-1
Manufacturer	Raybiotech
Amount	10 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Purity	>95% determined by SDS-PAGE
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	TFPI2,PP5,REF1
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Protein
Shipping Temperature	Gel Pack
Storage Conditions	The protein may be stored for 12 months at -20° C
Molecular Weight	This protein is fused with 6xHis tag at the N-terminus, has a calculated MW of 22.7 kDa. The predicted N-terminus is Asp 23. DTT-reduced Protein migrates as 26-32 kDa due to glycosylation.
Gene Symbols	TFPI2
Reconstitution	Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µ g/ml. Do not vortex. This solution can be stored at 2-8° C for up to 1 month. For extended storage, it is recommended to store at -20° C.
Endotoxin Level	<1 EU/ug by LAL method
Biological activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin, 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
Introduction	Tissue Factor Pathway Inhibitor 2 (TFPI2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF1), is a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). TFPI2 is highly abundant in the full-term placenta and widely expressed in umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. TFPI2 may play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa and has no effect on thrombin. Reduced synthesis of TFPI-2 has been related to numerous pathophysiological processes such as inflammation, angiogenesis, atherosclerosis, retinal degeneration and tumor growth / metastasis.
Country of Animal Origin	USA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.