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Recombinant Mouse M-CSF

Recombinant Mouse M-CSF

Manufacturer	Raybiotech
Category	Oncology Peptides & Proteins Recombinant Proteins By Organ Colorectal Cancer Lymphatic System Cancer
Type	Proteins
Specific against	Mouse
Amount	10 ug
Item no.	229-20305
eClass 6.1	34160400
eClass 9.0	42020190
Available
Short Description	Purified recombinant M-CSF expressed in E.coli
Introduction	M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells. Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.
Protein Name	M-CSF
Molecular Weight (kDa)	36.4kD (156 amino acid homodimer)
Purity	%98 by SDS PAGE and HPLC analysis
Concentration	ca.0.1mg/ml
Preservative	None present
Endotoxin Level	<1EU/ug
Protein Type	Recombinant Protein
Recommended Applications	Functional Assays
Biological activity	The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg.
Reconstitution	Reconstitute with 100ul distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.
Storage/stability	Prior to reconstitution store at +4° C. After reconstitution store at -20° C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.