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Recombinant Mouse interleukia IL-16 Protein

Recombinant Mouse interleukia IL-16 Protein

Item no.	230-30050-10
Manufacturer	Raybiotech
Amount	10 ug
Quantity options	10 ug 100 ug 250 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Type	Proteins
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	Mouse (Murine, Mus musculus)
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Animal Proteins
Manufacturer - Conjugate / Tag	N-terminal histidine tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein has a calculated molecular weight of about 14 kDa. Due to the post-translation modifications, it migrates as approximately 14-20 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein sizes slightly decreased. See deglycosylation analysis image below.
Description	Recombinant mouse interleukia IL-16, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	Il16
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Expressed Region	Ser1205-Ser1322
Protein Name & Synonyms	Pro-interleukin-16 [Cleaved into: Interleukin-16 (IL-16) (Lymphocyte chemoattractant factor) (LCF)], Interleukin 16, IL-16
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by Pierce BCA protein assay kit
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level	Not determined
Preservative	None
SDS-PAGE Image	Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein size and indicated the untreated recombinant protein (Lane 2) was glycosylated.Lane 1, protein standard ladder (kDa).Lane 2, untreated protein.Lane 3, treated protein with deglycosylation enzymes under native conditions.Lane 4, treated protein with deglycosylation enzymes under denature conditions.Lane 5, deglycosylation enzymes only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

230-30050-10.pdf

230-30050-10-datasheet.pdf

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Amount: 10 ug

available

Delivery expected until 10/16/2025

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