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Pfu-DNA Polymerase Recombinant

Pfu-DNA Polymerase Recombinant

Item no.	228-11794-3
Manufacturer	Raybiotech
Amount	2500 IU
Quantity options	500 IU 2500 IU
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Liquid
Applications	PCR, other
Specific against	other
Host	E.coli
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Similar products	Pfu-DNA
Available
Manufacturer - Applications	1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3' overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75°, C. 7. 95% active after 1-hour incubation at 98°, C.
Storage Conditions	Pfu DNA Polymerase although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles.
Formulation	50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol.
Protein Name & Synonyms	DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase.
Format	Sterile liquid formulation.
Buffers	Reaction Buffer: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM (NH4)2SO4, 100mM KC1, 1% Triton X-100, 1mg/ml BSA, 20mM MgSO4.
Instructions for Use	PCR Protocol: Add the following components to a amplify 1kb DNA template: 5 ul 10x buffer with MgSO4. 4 ul 2.5mM dNTPs. 1 ul Primers mix (10 uM each). 0.2 ul Pfu-DNA Polymerase. and 38 ul Water. Amplify using the following cycling parameters: Heat Soak: 1 cycle at 94°C/4 min. Denaturation: 30 cycles at 94°C/30 sec. Annealing: 30 cycles at 58°C /30 sec. Extension: 30 cycles at 72°C /90 sec. Final: 1 cycle at 72°C /5 min.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.