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Recombinant Human DNER Protein

Recombinant Human DNER Protein

Item no.	230-30104-100
Manufacturer	Raybiotech
Amount	100 ug
Quantity options	10 ug 100 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Type	Proteins Recombinant
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	N-terminal histidine tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant human DNER protein has a calculated molecular mass of 64 kDa. Due to the abundant glycosylation, it migrates as approximately 150 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Description	Recombinant human Delta and Notch-like epidermal growth factor-related receptor, derived from transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	DNER
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Expressed Region	Asn35-Tyr640
Protein Name & Synonyms	DNER, Delta/Notch Like EGF Repeat Containing, Bet, Delta-Notch-Like EGF Repeat-Containing Transmembrane, Delta/Notch-Like EGF Repeat Containing, WUGSC:H_NH0150O02.1, H_NH0150O02.1, UNQ26.
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by Pierce BCA protein assay kit
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

230-30104-100.pdf

230-30104-100-datasheet.pdf

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Amount: 100 ug

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Delivery expected until 10/16/2025

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