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Recombinant Human IgSF11 (N-Terminal) Protein

Recombinant Human IgSF11 (N-Terminal) Protein

Item no.	230-30108-200
Manufacturer	Raybiotech
Amount	200 ug
Quantity options	10 ug 200 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Type	Proteins Recombinant
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	N-terminal histidine tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant human IgSF11 protein has a calculated molecular mass of 24 kDa. Due to the abundant glycosylation, it migrates as approximately 30-35 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Description	Recombinant human immunoglobulin superfamily, member 11, derived from transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	IGSF11
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Expressed Region	Ala20-Gly244
Protein Name & Synonyms	IgSF11, N-terminal, Immunoglobulin Superfamily, Member 11, Brain And Testis-Specific Immunoglobulin Superfamily Protein, V-Set And Immunoglobulin Domain-Containing Protein 3, Cancer/Testis Antigen 119, BT-IgSF, CXADRL1, VSIG3, V-Set And Immunoglobulin Domain Containing 3, CXADR Like 1, IgSF13, BTIGSF, IgSF11, CT119
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by Pierce BCA protein assay kit
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

230-30108-200.pdf

230-30108-200-datasheet.pdf

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Amount: 200 ug

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Delivery expected until 10/16/2025

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