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Omega-conotoxin Bu8

Omega-conotoxin Bu8

Item no.	ALO-STC-180-0.5mg
Manufacturer	Alomone
Amount	0.5 mg
Quantity options	0.1 mg 0.5 mg 10 mg 1 mg 5 mg
Category	Metabolism Diabetes Reagents & Chemicals Other Chemicals
Type	Chemicals
Format	Lyophilized
Specific against	other
Purity	≥98% (HPLC)
Formula	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Sequence	CKRKGSSCRRTSYDCCTGSCRNGKC-NH<sub>2</sub>
ECLASS 10.1	32160000
ECLASS 11.0	32160000
UNSPSC	12000000
Shipping Condition	Room temperature
Available
Manufacturer - Type	Non Antibodies
Manufacturer - Applications	Electrophysiology
Manufacturer - Category	Proteins
Manufacturer - Targets	CaV2.2 Ca2+ channel
Country of Origin	Israel
Shipping Temperature	The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Storage Conditions	Storage as Solution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Molecular Weight	2753 Da
Manufacturer - Format	Lyophilized
Short description	A Specific Blocker of CaV2.2 Channel
Description	Bu8, Omega-conotoxin Bu8 - A Specific Blocker of CaV2.2 Channel
Reconstitution	Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Specificity	A Specific Blocker of Ca_V2.2 Channel
PH	7, 4
UNSPSC	12352202
Origin	Conus bullatus (Bubble cone)
Modifications	Disulfide bonds between: Cys¹-Cys¹⁶, Cys⁸-Cys²⁰, Cys¹⁵-Cys²⁵ Cys²⁵ - C-terminal amidation
Effective Concentration	10 - 100 nM
Activity	Specific and potent N-type voltage-gated calcium (CaV2.2) channel blocker1.
Solubility	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Sterile endotoxin free	no
Bioassay tested	yes
Scientific Background	ω-Conotoxin Bu8 (Bu8) is a 25 amino acid peptidyl toxin that was synthesized from the venom of the cone snail, Conus bullatus1. This toxin potently and selectively blocks N-type voltage-gated calcium (Cav2.2) channels. Bu8 displays higher or similar analgesic activity in the pain models compared to MVIIA but has less adverse effects1.Bu8 adopts a canonical globular scaffold, demonstrated as an inhibitory cysteine knot motif, marked by 3 disulfide bonds that is consistent for all ω-conotoxins in the all-crossing pattern of 1-4, 2-5, and 3-61.Cav2.2 are predominantly expressed in nerve terminals and are involved in the regulation of neuronal excitability and nociceptive signaling. These channels are multifunctional and play important roles in the transduction of acute and chronic pain perception. They are able to transduce electrical activity into other cellular functions, regulate calcium homeostasis, and process pain information. Since ω-conotoxins selectively inhibit Cav2.2 in nociceptors, they are considered attractive molecules for drug design2.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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ALO-STC-180-0.5mg-safety-datasheet.pdf