Comparison

DsbC Set

Item no. GFK-PF005-10ML-EX
Manufacturer Cosmobio
Amount 5 x 2 ml Equivalents
Quantity options 500 ul Equivalents 5 x 2 ml Equivalents 25 x 2 ml
Category
Type Substrate
Specific against other
ECLASS 10.1 42010290
ECLASS 11.0 42010290
UNSPSC 12000000
Available
Manufacturer - Category
Protein Engineering/Cell Free Synthesis/Reconstituted/Disulfide Bond Supplements
Maker
GFK
Short Description
DsbC Set is a supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote synthesis of functional proteins that contain disulfide bonds. \\DsbC Set comprises E.coli disulfide bond isomerase (DsbC), which catalyzes disulfide bridge exchange reactions, and GSSG (oxidized glutathione) to create an oxidizing environment.
Description
Redox Environment Supplements Promote Disulfide Bond Formation
Formation of disulfide bonds is important for folding and stability of secretory proteins such as enzymes or antibodies. Disulfide bonds are usually formed by oxidation of sulfhydryl groups (SH-) of adjacent cysteine residues. Therefore, disulfide bond formation efficiency depends on redox state. Additionally, disulfide bond isomerase which can catalyze the exchange of disulfide bridges may be required for correct cysteine pairing.
Redox Supplement
DsbC Set comprises highly purified E. coli DsbC (a disulfide bond isomerase) which can catalyze disulfide bridge exchange, and GSSG (oxidized Glutathione) to enforce an oxidized environment.
-
Reagent
Qty
Description
Storage
GSSG
12.5 ul
Oxidized glutathione (60 mM)
-20 C
DSBC
25 ul
E.coli DsbC (320 µ M *2) (No tags)
-80 C
Dilution Buffer
500ul
30% glycerol buffer
-20 C
Redox Supplement
PDI Set comprises oxidized glutathione (GSSG), human PDI (protein disulfide isomerase) and human Ero1α (ER oxidoreductin-1 to reoxidize PDI).
-
Reagent
Qty
Description
Storage
GSSG
12.5 ul
Oxidized glutathione (60 mM)
-20 C
PDI
25 ul
Human protein disulfide isomerase with no tags (200 µ M)
-80 C
Ero1a
25 ul
Human ER oxidoreductin-1 to reoxidize PDI with no tags (5 µ M)
-80 C
Dilution Buffer
500 ul
30% glycerol buffer
-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 x 2 ml Equivalents
Available: In stock
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