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Recombinant Anti-CD146/MCAM Antibody, Rabbit Monoclonal

Recombinant Anti-CD146/MCAM Antibody, Rabbit Monoclonal

Item no.	10115-R044-100
Manufacturer	Sino Biological
Amount	100 uL
Quantity options	1 mL 100 uL 20 uL 50 uL
Category	Neuroscience Neural Lineage Markers Neuroinflammation Neural Adhesion Molecules Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies Immunostaining Adhesion Molecule Markers Immunochemistry
Type	Antibody Monoclonal
Applications	IF, IHC, ICC, ELISA
Clone	44
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	10115-R044
Similar products	MCAM
Available
Manufacturer - Type	Antibody
Storage Conditions	This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Antigen molecule name	MCAM
Immunogen	Recombinant Human CD146 protein (Catalog#10115-H08H)
Purification	Protein A
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; Catalog#10115-H08H; NP_006491.2; Met 1-Gly 559).
Specificity	Human CD146 / MCAM
Buffer	0.2 um filtered solution in PBS with 5% trehalose
No cross-reactivity	No cross-reactivity in ELISA with Human CD66a / CEACAM1 Human CD166 / alcama Human CD106 / VCAM1 Human CD226 / DNAM-1 Human BCAM / CD239
ELISA	This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect Human CD146. The detection limit for Human CD146 is approximately 0.00245 ng/well.
Recommended ELISA Concentration	ELISA: 0.1-0.2 ug/mL
Recommended ICC-IF Concentration	ICC/IF: 5-20 ug/mL
Recommended FCM Concentration	FCM: 0.5-2 ug/Test
Recommended IHC-P Concentration	IHC-P: 1-5 ug/mL

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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