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Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal

Item no.	10351-MM01-200
Manufacturer	Sino Biological
Amount	200 uL
Category	Antibodies Immunoprecipitation Western Blot ELISA Primary Antibodies
Type	Antibody Monoclonal
Applications	WB, IP, ELISA
Clone	4E 8H3
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	sino
Available
Antigen molecule name	HSPB1
Storage	This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunogen	Recombinant Human HSP27 Protein (Catalog#10351-H08E)
Purification	Protein A
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Specificity	Human HSP27
No cross-reactivity	No cross-reactivity with E.coli cell lysate in ELISA.
Buffer	0.2 um filtered solution in PBS with 5% trehalose
ELISA	This antibody can be used at 0.5-1 ug/mL with the appropriate secondary reagents to detect HSP27. The detection limit for HSP27 is approximately 0.0195 ng/well.
Recommended WB Concentration	WB: 2-10 ug/mL
Recommended ELISA Concentration	ELISA: 0.5-1 ug/mL
Recommended IP Concentration	IP: 4-6 ug/mg of lysate

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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