Influenza A H5N1 (Avian Flu) Hemagglutinin / HA ELISA Pair Set

Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.

Streptavidin-HRP: Store at 4C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

This H5N1 HA / Hemagglutinin ELISA Pair Set (SEK002) can quantificationally detect Anhui-HA (A/Anhui/1/2005), Indonesia-HA (A/Indonesia/5/2005), and Vietnam-HA (A/Vietnam/1194/2004). And it can also react with Turkey-HA (A/turkey/Turkey/1/2005) and Qinghai-HA (A/bar-headed goose/Qinghai/14/2008).

0.5 mg/mL biotinylated rabbit anti-H5N1 hemagglutinin polyclonal antibody. Dilute to a working concentration of 1.5 ug/mL in detection antibody dilution buffer before use.

The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of hemagglutinin.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any hemagglutinin present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-hemagglutinin polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of hemagglutinin present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.