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Human DKK3 / Dkk-3 / REIC ELISA Pair Set

Human DKK3 / Dkk-3 / REIC ELISA Pair Set

Item no.	SEK10203-5
Manufacturer	Sino Biological
Amount	5 Plates
Category	Neuroscience Neural Lineage Markers Antibodies ELISA Immunoassay Glial Cell Markers Antibody Pairs
Type	ELISA Pair Set
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10203
Similar products	DKK3
Available
Molecule	DKK3
Storage	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Conjugate	Biotin
Capture Ab	0.5 mg/mL of mouse anti-DKK3 monoclonal antibody. Dilute to a working concentration of 2 ug/mL in CBS before coating.
Detection Ab	Each vial contains 120 ug biotinylated rabbit anti-DKK3 polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 98 ng of recombinant DKK3. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Streptavidin-HRP	50 uL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Sensitivity	The minimum detectable dose of human Dkk3 / REIC was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test	The human Dkk3 / REIC ELISA Pair Set is for the quantitative determination of human Dkk3. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Dkk3 /REIC coated on a 96-well plate. Standards and samples are added to the wells, and any Dkk3 / REIC present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-Dkk3 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of Dkk3 / REIC present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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