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Human LAYN Matched ELISA Antibody Pair Set

Human LAYN Matched ELISA Antibody Pair Set

Item no.	SEK10208-5
Manufacturer	Sino Biological
Amount	5 Plates
Quantity options	15 Plates 5 Plates
Category	Antibodies ELISA Immunoassay Immunostaining Antibody Pairs
Type	ELISA Pair Set
Applications	ELISA
Specific against	Human (Homo sapiens)
Conjugate/Tag	HRP
Sensitivity	The minimum detectable dose of Human Layilin / LAYN was determined to be approximately <strong>15.625 pg/ml</strong>. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10208
Similar products	LAYN
Available
Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	LAYN
Capture Ab	0.5 mg/mL of mouse anti-Human Layilin / LAYN monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in PBS before coating.
Detection Ab	0.2 mg/mL of mouse anti-Human Layilin / LAYN monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 100 ng of recombinant Human Layilin / LAYN. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Principle of the test	The Human Layilin / LAYN ELISA Pair Set is for the quantitative determination of Human Layilin / LAYN. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Layilin / LAYN coated on a 96-well plate. Standards and samples are added to the wells, and any Human Layilin / LAYN present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human Layilin / LAYN monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Layilin / LAYN present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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