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Recombinant Canine EGFR Protein (His Tag), HPLC-verified

Recombinant Canine EGFR Protein (His Tag), HPLC-verified

Item no.	70026-D08B-20
Manufacturer	Sino Biological
Amount	20 ug
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Oncology Neuroscience Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Astrocytoma By Organ Bladder Cancer
Type	Proteins
Specific against	Dog (Canine, Canis lupus familiaris)
Conjugate/Tag	HIS
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Ser618
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	70026-D08B
Similar products	EGFR
Available
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	EGFR
Protein Construction	A DNA sequence encoding the canine EGFR (XP_533073.3) (Met1-Ser618) was expressed with a C-terminal polyhistidine tag.
Expressed Host	Baculovirus-Insect cells
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized canine EGFR-His at 10 ug/ml (100 ul/well) can bind biotinylated canine His-EGF(Cat:70013-D07E), The EC50 of biotinylated canine His-EGF(Cat:70013-D07E) is 0.16-0.36ug/ml
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant canine EGFR comprises 629 amino acids and has a predicted molecular mass of 69.8 kDa. The apparent molecular mass of the protein is approximately 87.2 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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