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Recombinant Human Carbonic Anhydrase IX/CA9 Protein (ECD, His Tag), HPLC-verified

Recombinant Human Carbonic Anhydrase IX/CA9 Protein (ECD, His Tag), HPLC-verified

Item no.	10107-H08H-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 10 ug 100 ug 2 mg 20 ug 4 mg
Category	Oncology Neuroscience Molecular Targets for Neuroscience By Cell Type Blastoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Asp414
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CAIX,Carbonic Anhydrase IX,MN
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CA9
Protein Construction	A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	Measured by its esterase activity. The specific activity is >30 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 ug enzyme at 400 nm in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Gln 38
Molecule Mass	The recombinant human CA9 consists of 388 amino acids and predicts a molecular mass of 42.5 kDa. As a result of glycosylation, rhCA9 migrates with apparent molecular mass of 48 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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