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Recombinant Human CD25/IL-2R alpha Protein (ECD, His Tag), HPLC-verified

Recombinant Human CD25/IL-2R alpha Protein (ECD, His Tag), HPLC-verified

Item no.	10165-H08H-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg 50 ug
Category	Oncology Neuroscience Neural Lineage Markers By Cell Type Hematology Leukemia Lymphoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Purity	> 97 % as determined by SDS-PAGE
Sequence	Met1-Cys213
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD25,IDDM10,IL-2RA,IL2R,p55,TCGFR
Similar products	IL2RA
Available
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	IL2RA
Protein Construction	A DNA sequence encoding the extracellular domainof human IL2 receptor alpha (NP_000408.1) (Met 1-Cys 213) was expressed with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Bio-Activity	1. Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his (Cat:10165-H08H) bound to Human IL2 was 35nM. 2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED50 for this effect is 0.15-0.75ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 22
Molecule Mass	The recombinant human IL2Ralpha consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Ralpha is approximately 44 kDa due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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