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Recombinant Human DKK-1 Protein (His Tag), HPLC-verified

Recombinant Human DKK-1 Protein (His Tag), HPLC-verified

Item no.	10170-H08H-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg 5 ug
Category	Oncology Neuroscience Molecular Targets for Neuroscience By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met2-His266
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	DKK-1,SK
Similar products	DKK-1, SK
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	DKK1
Protein Construction	A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Bio-Activity	Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 ug/ml in the presence of 10 ng/mL of mouse Wnt3a.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Thr 32 or Ser 35
Molecule Mass	The recombinant human DKK1 protein consists of 235 amino acids and has a calculated molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 45 kDa due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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