Comparison

Recombinant Human E-Cadherin Protein (ECD, hFc Tag), HPLC-verified European Partner

Item no. 10204-H02H-50
Manufacturer Sino Biological
Amount 50 ug
Quantity options 1 mg 100 ug 2 mg 4 mg 50 ug
Category
Type Proteins
Specific against Human (Homo sapiens)
Host Human
Conjugate/Tag Fc
Purity > 90 % as determined by SDS-PAGE
Sequence Met1-Ile707
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Arc-1,CD324,CDHE,E-cad,E-Cadherin,ECAD,LCAM,UVO
Similar products CDH1
Available
Manufacturer - Conjugate / Tag
C-human IgG1-Fc
Shipping Temperature
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name
CDH1
Protein Construction
A DNA sequence encoding the human E-Cad (P12830)(Met1-Ile707) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host
Human Cells
Bio-Activity
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.
When cells are added to E-Cad coated plates (5 ug/mL, 100 uL/well), approximately 33% will adhere specifically after 90 minutes at 37 C.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 23 & Asp 155
Molecule Mass
The recombinant human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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