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Recombinant Human CD40 Ligand/TNFSF5 Protein (His Tag), HPLC-verified

Recombinant Human CD40 Ligand/TNFSF5 Protein (His Tag), HPLC-verified

Item no.	10239-H08E-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Category	Neuroscience Neural Lineage Markers Aging & Neurological Disorders Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Glu108-Leu261
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD154,CD40 Ligand,CD40L,gp39,hCD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP
Similar products	CD40LG
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CD40LG
Protein Construction	A DNA sequence encoding the soluble form of human CD40L (NP_000065.1) (Glu 108-Leu 261) was expressed with an initial Met at the N-terminus and a polyhistide tag at the C-terminus.
Expressed Host	E.coli
Bio-Activity	Measured by its binding ability in a functional ELISA. 1. Immobilized human CD40 at 2 ug/ml (100 ul/well) can bind biotinylated human CD40L with a linear range of 15.6-500 ng/ml. 2. Immobilized human CD40 / Fc at 2 ug/ml (100 ul/well) can bind biotinylated human CD40L with a linear range of 7.8-125 ng/ml.
Endotoxin	Please contact us for more information.
Predicted N Terminal	Met
Molecule Mass	The recombinant human CD40L consists of 161 amino acids and has a calculated molecular mass of 17.7 kDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4, 10% glycerol

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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