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Recombinant Human Serpin A1/Alpha-1-antitrypsin Protein (His Tag), HPLC-verified

Recombinant Human Serpin A1/Alpha-1-antitrypsin Protein (His Tag), HPLC-verified

Item no.	10306-H08H-20
Manufacturer	Sino Biological
Amount	20 ug
Quantity options	1 mg 10 ug 100 ug 2 mg 20 ug 4 mg
Category	Peptides & Proteins Recombinant Proteins Cardiovascular Research Hypertension
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
Conjugate/Tag	HIS
Purity	> 97 % as determined by SDS-PAGE
Sequence	Met1-Lys418
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	A1A,A1AT,AAT,alpha1AT,MGC23330,MGC9222,PI,PI1,PRO2275,SerpinA1
Similar products	SERPINA1
Available
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	SerpinA1
Protein Construction	A DNA sequence encoding the human SerpinA1 (NP_000286.3) pre-protein (Met 1-Lys 418) was expressed with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Bio-Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 3.0 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T1426), 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 25
Molecule Mass	The secreted mature form of recombinant human SerpinA1 consists of 405 amino acids and has a calculated molecular mass of 45.7 kDa. Due to glycosylation, the rhSerpinA1 migrates as an approximately 55-60 kDa protein in SDS-PAGE analysis under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10306-H08H-20-datasheet.pdf

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Amount: 20 ug

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