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Recombinant Human HMGB1 Protein (His Tag), HPLC-verified

Recombinant Human HMGB1 Protein (His Tag), HPLC-verified

Item no.	10326-H08H-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 100 ug 2 mg 4 mg 500 ug
Category	Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders Neural Signaling Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	HMG-1, HMG1, HMG3, SBP-1
Similar products	HMGB1
Available
Manufacturer - Category	Protein
Molecule	HMGB1
Synonym	HMG-1, HMG1, HMG3, SBP-1
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Expressed Host	Human Cells
Purity	> 93 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA . Immobilized human HMGB at 2 ug/ml (100 ul/well) can bind human AGER. The EC50 of human AGER is 0.27 ug/ml .
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met 1
Molecule Mass	The recombinant human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_002119.1
Amino acid sequence	Met1-Glu215
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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