Comparison

Recombinant Human Sonic Hedgehog/SHH Protein (aa 198-462, His Tag) European Partner

Item no. 10372-H08H-5
Manufacturer Sino Biological
Amount 5 ug
Quantity options 1 mg 100 ug 2 mg 20 ug 4 mg 5 ug
Category
Type Proteins
Specific against Human (Homo sapiens)
Conjugate/Tag HIS
Purity > 97 % as determined by SDS-PAGE
Sequence Met1-Gly197
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias HHG1,HLP3,HPE3,MCOPCB5,SMMCI,Sonic hedgehog,TPT,TPTPS
Similar products SHH
Available
Manufacturer - Conjugate / Tag
C-His
Shipping Temperature
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name
SHH
Protein Construction
A DNA sequence encoding the amino acid sequence (Cys 198-Ser 462) of human SHH (Q15465), that is Sonic hedgehog protein C-product, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Expressed Host
Human Cells
Bio-Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse fibroblasts.
The ED50 for this effect is 12-24 ug/mL.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Cys 198
Molecule Mass
The recombinant human SHH( aa 198-462) consists of 276 amino acids and has a predicted molecular mass of 29 kDa. The apparent molecular mass of the protein is approximately 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 ug
Available: In stock
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