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Human TFPI Protein (His Tag)

Human TFPI Protein (His Tag)

Manufacturer	Sino Biological
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Format	Human Cell
Amount	10ug
Host	Human
Item no.	10564-H08H-10
eClass 6.1	34160400
eClass 9.0	42020190
Available
Molecule	TFPI
Synonym	EPI, LACI, TFI, TFPI1
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) was expressed with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100uL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 uM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5 .
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Asp 29
Molecule Mass	The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_006278.1
Amino acid sequence	Met1-Lys282
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10ug

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Delivery expected until 5/23/2024