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Recombinant Human TNF-alpha Protein, HPLC-verified

Recombinant Human TNF-alpha Protein, HPLC-verified

Item no.	10602-HNAE-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 10 ug 100 ug 2 mg 4 mg 50 ug 500 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins
Specific against	Human (Homo sapiens)
Host	E.coli
Purity	> 95 % as determined by SDS-PAGE
Sequence	Val77-Leu233
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	DIF,TNF-alpha,TNFA,TNFSF2
Similar products	TNF
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	Native
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	TNF
Protein Construction	A DNA sequence encoding the human TNF-alpha soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Expressed Host	E.coli
Bio-Activity	Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-100 pg/ml, corresponding to a specific activity of 3 x 10E6-2 x 10E7 units/mg.
Endotoxin	Please contact us for more information.
Predicted N Terminal	Met
Molecule Mass	The recombinant human TNF-alpha consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10602-HNAE-100-datasheet.pdf

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Amount: 100 ug

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