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Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

Item no.	10692-H20B1-20
Manufacturer	Sino Biological
Amount	20 ug
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Targets for Rodent Neurosciences By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Mesothelioma Astrocyte Markers
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	GST, HIS
Purity	> 97 % as determined by SDS-PAGE
Sequence	Lys956-Ser1390
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	AUTS9,c-Met,DFNB97,HGFR,RCCP2
Available
Manufacturer - Conjugate / Tag	N-GST & His
Shipping Temperature	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	MET
Protein Construction	A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) (Cat: 10692-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human HGF-his (Cat: 10463-H08H) with a linear range of 15.6-125 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human MET /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10692-H20B1-20-datasheet.pdf

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