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Recombinant Human Transferrin Protein (His Tag), HPLC-verified

Recombinant Human Transferrin Protein (His Tag), HPLC-verified

Item no.	11019-H08H-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Category	Neuroscience Targets for Rodent Neurosciences Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
Conjugate/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Pro698
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	HEL-S-71p,PRO1557,PRO2086,TFQTL1,Transferrin
Similar products	TF
Available
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	TF
Protein Construction	A DNA sequence encoding the human transferrin (NP_001054.1) (Met 1-Pro 698) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized human CD71 at 10 ug/ml (100 ul/well) can bind human Transferrin. The EC50 of human Transferrin is 5.6 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Val 20
Molecule Mass	The secreted recombinant human transferrin comprises 690 amino acids with a predicted molecular mass of 76.6 kDa. It migrates as an approximately 74 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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