Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag), HPLC-verified

C-His

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met 1-Ala 263) was expressed, fused with a polyhistidine tag at the C-terminus.

1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 ug/ml (100 ul/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 ug/ml (100 ul/well) can bind mouse CD36 with a linear ranger of 6.4-800 ng/ml.
3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.05-0.5 ug/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.

Ser 21

Lyophilized from sterile PBS, pH 7.4