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Recombinant Human TGF-alpha Protein, HPLC-verified

Recombinant Human TGF-alpha Protein, HPLC-verified

Item no.	11252-HNAE-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg
Category	Oncology Neuroscience Neural Signaling By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	E.coli
Specific against	Human (Homo sapiens)
Host	E.coli
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	TFGA, TGFa
Similar products	TGFA
Available
Manufacturer - Category	Protein
Molecule	TGFA
Synonym	TFGA, TGFa
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.
Expressed Host	E.coli
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized human TGFa (cat:11252-HNAE) at 10 ug/ml (100 ul/well) can bind human EGFR-Fc (Cat:10001-H02H). The EC50 of human EGFR-Fc (Cat:10001-H02H) is 20-60 ng/ml. 2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-0.5ng/mL.
Endotoxin	Please contact us for more information.
Predicted N Terminal	Met
Molecule Mass	The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0.
Accession	P01135-1
Amino acid sequence	Val40-Ala89
TAG	Native

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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