Recombinant Human beta-Catenin Protein (His & GST Tag)

N-GST & His

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the human CTNNB1 (P35222-1) (Met 1-Leu 781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

< 1.0 EU per ug of the protein as determined by the LAL method

The recombinant human CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.

Catenin beta-1, also known as CTNNB1, is a cytoplasm and nucleus protein which belongs to thebeta-catenin family. Catenin beta-1 / CTNNB1 contains 12ARM repeats. Catenin beta-1 / CTNNB1 is expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Catenin beta-1 / CTNNB1 is also expressed in colon. Catenin beta-1 / CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Catenin beta-1 / CTNNB1 is involved in signal transduction through the Wnt pathway. Nuclear beta-catenin is involved in transcriptional regulation by association with transcription factors of the TCF / LEF family. Defects in CTNNB1 are associated with colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB) susceptibility to ovarian cancer (OC).

Recombinant Human CTNNB1 protein

5% Mannitol, 5% Trehalose, 0.02% Tween80