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Recombinant Human RANKL/TNFSF11 Protein (ECD, hFc Tag)

Recombinant Human RANKL/TNFSF11 Protein (ECD, hFc Tag)

Item no.	11682-H01H-5
Manufacturer	Sino Biological
Amount	5 ug
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg 5 ug 50 ug 500 ug
Category	Oncology Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines By Organ Lymphatic System Cancer
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	Fc
Purity	> 86 % as determined by SDS-PAGE
Sequence	Gly63-Asp244
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD254,hRANKL2,ODF,OPGL,OPTB2,RANKL,sOdf,TRANCE
Similar products	TNFSF11
Available
Manufacturer - Conjugate / Tag	N-human IgG1-Fc
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	TNFSF11
Protein Construction	A DNA sequence encoding the human TNFSF11 isoform 2 (O14788-2) (Gly 63-Asp 244) was fused with the Fc region of human IgG1 at the N-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF11B-His (Cat?10271-H08H) at 10 ug/ml (100 ul/well) can bind human Fc-TNFSF11 (Cat?11682-H01H) with a linear ranger of 3.125-200 ng/mL. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 0.1-0.4 ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 20
Molecule Mass	The recombinant human TNFSF11/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 443 amino acids and has a calculated molecular mass of 48.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFSF11/Fc monomer is approximately 50-55 kDa due to the glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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