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Recombinant Human IRE1 Protein (aa 465-977), HPLC-verified

Recombinant Human IRE1 Protein (aa 465-977), HPLC-verified

Item no.	11905-HNCB-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Baculovirus-Insect cells
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	hIRE1p, IRE1, IRE1a, IRE1P
Similar products	ERN1
Shipping Condition	Cool pack
Available
Manufacturer - Category	Protein
Shipping method	Blue ice shipping
Molecule	ERN1
Synonym	hIRE1p, IRE1, IRE1a, IRE1P
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 80 % as determined by SDS-PAGE
Bio-Activity	1. Kinase activity untested 2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Gly
Molecule Mass	The secreted recombinant human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.
Accession	O75460-1
Amino acid sequence	Pro465-Leu977
TAG	N-cleavage

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ug

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