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Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (His & GST Tag)

Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (His & GST Tag)

Item no.	CT008-H0907B-20
Manufacturer	Sino Biological
Amount	20 ug
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Oncology Metabolism By Cell Type Hematology Lymphoma Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Status Autophagy
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
Conjugate/Tag	GST, HIS
Purity	> 94 % as determined by SDS-PAGE
Sequence	Met1-Pro331 & Met1-Ile272 & Met1-Arg552
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	5' adenosine monophosphate-activated protein kinas,5' AMP-activated protein kinase,AMPK,beta 1 non-catalytic subunit and gamma 1 non-catal
Similar products	AMPK
Available
Manufacturer - Conjugate / Tag	N-GST & N-His
Shipping Temperature	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	AMPK
Protein Construction	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met & Met & His
Molecule Mass	The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 40, 42 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CT008-H0907B-20-datasheet.pdf

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Amount: 20 ug

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